Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Immunohistochemistry S-P method

YB Yanqing Bi
MT Mengzi Tian
JL Jinghong Le
LW Linlin Wang
XL Xiaofang Liu
JQ Jianhua Qu
MH Min Hao
ask Ask a question
Favorite

For this study, we used the following: rabbit IgG (Nanjing Kaiji Company) and peroxidase-labeled goat anti-rabbit IgG (Shenzhen Jinmei Biological Co., Ltd.), antibody dilutions (Wuhan Boshide Biotechnology Company), constitutive rafter acid salt buffer liquid powder (Nanjing Kaiji Company), and neutral gum (Shanghai Specimen Model Factory). Paraffin sections made from a tissue-slicing machine (Leica, Germany) were dewaxed in water; 3 % H2O2 was incubated at room temperature for 5 ~ 10 min, to eliminate endogenous peroxidase activity. The S-P immunohistochemical reagent kit was provided by Fujian Maixin Biotechnology Company.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A