Measurement of trans-endothelial electrical resistance (TEER)

KK Korakoch Kangwantas
EP Emmanuel Pinteaux
JP Jeffrey Penny
request Request a Protocol
ask Ask a question
Favorite

An EVOM™ Epithelial Voltohmmeter and chopstick electrodes (World Precision Instruments, UK) were used for the measurement of the electrical resistance of brain endothelial cell monolayers grown on Transwell® inserts. Monolayer TEER was calculated by subtracting the value of a blank Transwell® insert from the total resistance obtained for the endothelial monolayer and insert, and each TEER value was normalised for the surface area of the insert.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A