The HL-60 cell line is derived from human promyelocytic leukemia and can be differentiated into neutrophil-like cells upon incubation with DMSO [24]. HL-60 cells were kept in RPMI 1640 media (supplemented with 10% FCS) with 1.5% DMSO (Sigma Aldrich, St. Louis MO) for the indicated number of days.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.