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GFP-Bax HCT 116 cells were subject to either Mock- or VPS35-siRNA treatment. In the last 60 min of the siRNA treatment, the cells were treated with 1 μM staurosporine (Sigma Aldrich). Cells were immunostained with anti-Tom20 as previously described. 250 cells per treatment were designated as either having inactive (cytoplasmic) Bax or active (punctate) Bax.
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