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Cellular uptake analyzed by flow cytometry analysis
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To understand the targeting effect of polyprodrug nanoreactors, 4T1 cells were seeded on 6-well plates at a density of 1 × 105, respectively. After incubation for 24 h, the cells were treated with various samples and incubated for 6 h at 37 °C. Then, the cells were washed with cold PBS, harvested and analyzed immediately using flow cytometry (Cytomics FC 500, Beckman Coulter, USA). The mean fluorescence intensity of 1 × 104 cells was recorded for each sample.
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