siRNA transfection was performed using a reverse transfection method with lipofectamine 3000 (Thermo Fisher, Waltham, MA). Non-targeting (Scrambled; D-001810-01-05), TFAP2A (L-006348-02-0005), TFAP2C (L-005238-00-0005), and PPARG (L-003436-00) siRNAs were obtained from Dharmacon (Lafayette, CO). siRNA and lipofectamine 3000 were added to separate aliquots of OPTi-MEM medium, respectively, and incubated for 5 min. Subsequently, siRNA and lipofectamine 3000 in OPTi-MEM were mixed and incubated for 20 min. After incubation, siRNA-lipofectamine 3000 complex was added to 2 ml cell suspension containing 4 × 105 target cells per well in a 6-well plate.
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