Melanoma xenografts were created in C57BL/6J mice by injecting subcutaneously 5 × 104 B16F10 melanoma cells into the left flank. Experiments were performed after tumors became palpable, 8–10 days after the injection.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.