Immunblotting (IB)

ND Neerajana Datta
SI Saimul Islam
UC Uttara Chatterjee
SC Sandip Chatterjee
CP Chinmay K. Panda
MG Mrinal K. Ghosh
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Preparation of whole cell lysates and IB analyses were performed as described before57. The following primary antibodies were used: ERβ (sc-8974), PML (sc-5621), p21 (sc-53870), Actin (sc-1616), and GFP (sc-9996) (SantaCruz Biotechnology); Foxo3a (ab53287), p-Foxo3a (S253)(ab31109) (Abcam); Survivin (#2808), Bim (#2819), Bax (#2772), cleaved PARP (#9625) and cleaved Caspase 3 (#9664) (Cell Signalling Technology); HRP-tagged anti-rabbit and anti-mouse secondary antibodies (Cell Signalling Technology); HRP-tagged anti-goat secondary antibody (Sigma-Aldrich). Densitometry values of the immunoblots were computed using GelQuant.Net software.

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