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Spontanous pain was observed as licking, lifting and/or flinching of the affected hind paw. Mice were placed in individual polyvinyl circular containers (25 cm in diameter) on a surface padded with some tissue paper at room temperature. Mice were allowed to roam freely and spontaneous pain was measured in 5-min intervals, as counted by a blinded observer.
Copyright and License information: The Author(s) ©2016
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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