Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Phenotypic evolution of clonotype subsets.

AC A.G. Chapuis
DE D. N. Egan
MB M. Bar
TS T. M. Schmitt
MM M. S. McAfee
KP K. G. Paulson
VV V. Voillet
RG R. Gottardo
GR G. B. Ragnarsson
MB M. Bleakley
CY C. C. Yeung
PM P. Muhlhauser
HN H. N. Nguyen
LK L. A. Kropp
LC L. Castelli
FW F. Wagener
DH D. Hunter
ML M. Lindberg
KC K. Cohen
AS A. Seese
MM M. J. McElrath
ND N. Duerkopp
TG T. A. Gooley
PG P. D. Greenberg
request Request a Protocol
ask Ask a question
Favorite

To determine whether the individual infused clonotypes with TEM characteristics had evolved in vivo to include alternate subsets (TCM or TTD), PBMC obtained at ~100 days from Pts. 10, 11, 12 and 24, who were selected based on TTCR-C4 persistence and sample availability, were flow-sorted for TEM, TCM or TTD tet+ cells, and the clonotypes comprising each subset were determined.

Copyright and License information:  ©2019

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A