MTT assay

32D cells were plated in triplicate at a density of 3 × 10⁴ cells/well in a 96-well plate after washing twice with PBS. The JAK inhibitor ruxolitinib was added to the medium (max. vol. of 100 μl) at the concentration of 1 μM. Controls were treated with DMSO. Cells were cultured in WEHI-free RPMI medium. The measurement of cell viability was performed 48 h later using 10 μl 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (5 mg/ml H₂O) per well. The 96-well plate was incubated in the dark at room temperature for 4 h, and 100 μl isopropanol-HCl solution per well was added. Samples were analyzed with a microplate reader at a wavelength of 550 nm (Kayto, RT-2100C).