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4.3. Exosome Purification

NC Nicola Cuscino
LR Lavinia Raimondi
AL Angela De Luca
CC Claudia Carcione
GR Giovanna Russelli
LC Laura Conti
JB Jacopo Baldi
PC Pier Giulio Conaldi
GG Gianluca Giavaresi
AG Alessia Gallo
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Exosomes released by OS cells (SAOS-2, MG-63, and U-2 OS) during a 48-hour culture period were isolated from conditioned culture medium supplemented with 10% FBS, previously ultra-centrifuged by differential centrifugations as previously described. Exosome protein content was determined by the Bradford assay [16]. Exosomes from plasma of OS patients were isolated with Total Exosome Isolation Kit (Thermo Fisher Scientific, Cambridge, MA, USA), according to the manufacturer’s instructions.

Copyright and License information:  ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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