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Measurement of Blood Urea Nitrogen (BUN) and Serum Creatinine (SCr)
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At the time of mice sacrifice, blood samples were collected and kept at room temperature undisturbed for 30 min to enable blood clotting. Subsequently, blood samples were centrifuged at 2,000 × g for 10 min at 4°C and sera (supernatants) were subsequently collected. BUN and SCr concentrations in murine sera were determined using a Hitachi 7060 automated Chemistry Analyzer (Diamond Diagnostics, Inc.) according to the manufacturer's protocols.
Copyright and License information: ©2019 Peng et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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