Genome Sequencing and Annotation

YR Yi Ren
AP Agata Palusiak
WW Wei Wang
YW Yi Wang
XL Xiao Li
HW Huiting Wei
QK Qingke Kong
AR Antoni Rozalski
ZY Zhi Yao
QW Quan Wang
request Request a Protocol
ask Ask a question
Favorite

The paired-end reads generated using an Illimina HiSeq 2000 platform were assembled by SOAP de novo (Li et al., 2010). Coding sequences were predicted by Glimmer 3.0 (Delcher et al., 1999). Functional annotation was based on BLASTp, with the KEGG, Swiss-Prot, COG, and non-redundant GenBank CDS databases (Altschul et al., 1997; Kanehisa and Goto, 2000; Muller et al., 2010).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A