Mitochondrial morphology.

The SJ4103 strain was used to visualize mitochondria in the body wall muscle of L3 larvae. Worms were subjected to L3 arrest as described above. Starved worms were sampled over time, and representative images were taken with a Hamamatsu ORCA R² camera and Yokogawa spinning disk head mounted on a Zeiss AxioImager A1 microscope. The images were analyzed using IMARIS (Bitplane), and three-dimensional reconstructions were built from Z-stacks. Objects identified by the program were assessed for surface area, volume, and sphericity according to the default algorithms of the program.