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Mice were perfused transcardially with 4% paraformaldehyde (PFA) for light microscopy. Tissue was extracted and postfixed for up to 4 h at 4℃. Immunohistochemistry was performed on Vibratome (50 µm) sections using standard reagents and the following antibodies: anti-NK1 (1:10,000, Chemicon, Temecula, CA), anti-NeuN (1:500, Millipore).
Copyright and License information: The Author(s) ©2016
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
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