All stocks and crosses were raised on standard cornmeal/dextrose medium at 25°C in a 12/12 light/dark cycle. Canton S. served as the control strain (Woodard et al., 1989). Df (X) ten-a was used as a ten-a null mutant (Mosca et al., 2012). Mef2-GAL4 was used to drive expression in all muscles (Lilly et al., 1995). SG18.1-GAL4 was used to drive expression in all ORNs (Shyamala and Chopra, 1999). We also used the transgenic strains UAS-Ten-a (Mosca et al., 2012) and UAS-ten-mRNAi-V 51173 (Mosca et al., 2012) for Ten-a expression and ten-m RNAi knockdown, respectively.
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