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In this study, genomic DNA samples of all subjects were segregated by phenol chloroform methods. Taqman allelic discrimination assay was used to estimate SNP genotyping. PCR Taqman primers and probes were supplied by Applied Biosystems. The results were obtained by ABI 7500 Fast Real-time PCR System with the Sequence Detection Software. Gene magnification was heated at 95°C for 10 min, 53 cycles at 92°C for 30s and 60°C for 1 min. To maintain accuracy of analysis results, ten percent of samples were duplicated and the results were coherent.
Copyright and License information: ©2018 Tian et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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