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2.5. Annexin V‐FITC apoptosis detection
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PC3 cells were grown in 60‐mm dish to 60–70% confluency before treatment with sublethal concentration (5 μM) of ADAADi for 48 hr. The cells were stained with Annexin V and Propidium iodide using Annexin V‐FITC Apoptosis Detection Kit (Catalog # BMS500FI/100; eBiosciences/Thermo Fisher India, Mumbai, India). The samples were analyzed using BD FACS Calibur 4C flow cytometer (Becton Dickinson India, Gurgaon, India).
Copyright and License information: ©2019 The Authors. Published by Wiley Periodicals, Inc.
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