4.2. Cell Culture

Human prostate cancer (DU145: human epithelial cells- ATCC^® HTB-81TM) purchased from ATCC (Manassas, VA, USA) and lung cancer (A549: human epithelial cells- ATCC^® CCL-185TM) cells, kindly donated by Laboratory Medicine Department, RMIT University, were used in this study.

DU145 cells were cultured and maintained in MEM Alpha + GlutaMAX^TM and 15 mM HEPES (Gibco^®, Grand Island, NY, USA), 10% foetal bovine serum (FBS) (Gibco^®) and 1% Penicillin-Streptomycin (Gibco^®). A549 cells were cultured and maintained in DMEM/F12 supplemented with L-Glutamine and 15 mM HEPES (Gibco^®), 10% FBS (Gibco^®) and 1% Penicillin-Streptomycin (Gibco^®).

Both cell lines initially were cultured and grown to 80% confluence in a 25 cm² flask and then were sub-cultured. Incubation conditions during the experiments was 37 °C with 5% CO₂ in a humidified environment.