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Immunomagnetic cell isolation of various sub-populations of CB and PB experiments

AS Arjun Saha
SP Sachit Patel
LX Li Xu
PS Paula Scotland
JS Jonathan Schwartzman
AF Anthony J. Filiano
JK Joanne Kurtzberg
AB Andrew E. Balber
CB Cesar V Borlongan
CB Cesar V Borlongan
CB Cesar V Borlongan
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Specific sub-populations were isolated or removed from CB-MNC or PB-MNC by immunomagnetic sorting using EasySep cell kits for human CD34+, CD3+, CD14+ and CD19+ cells (Stemcell Technologies, Vancouver, Canada, Catalog #18096, #18051, #18058 and #18054 respectively) following the manufacturer’s directions. Flow-through fractions from positive selection columns were re-run through the columns to increase the purity of targeted populations. A sample of each cell preparation was analyzed by flow cytometry to determine cellular composition [28]. Immuno-magnetically selected specific sub-population of cells with ≥80% purity was used for any experiment. More highly purified populations were obtained by cell sorting as described below for gene expression analysis.

Copyright and License information:  ©2019 Saha et al ©2019 Cesar V BorlonganThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2019 Cesar V BorlonganThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2019 Cesar V BorlonganThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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