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Carbohydrate composition

LW Lynn M. Wendt
CK Christopher Kinchin
BW Bradley D. Wahlen
RD Ryan Davis
TD Thomas A. Dempster
HG Henri Gerken
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Total carbohydrate content of unstored and stored (composite of triplicate biological replicates) algae biomass was determined by the two-step process described in Van Wychen et al. [41], with all samples measured in duplicate. A composite of triplicate storage experiments was formed using lyophilized algae biomass, and an exception to this occurred in the anaerobic, 30-day storage sample, where all triplicate storage experiments were analyzed. Carbohydrates were first hydrolyzed with 72% sulfuric acid, diluted, and autoclaved at 121 °C for 60 min. Duplicate samples were quantified for monomeric sugars by high-performance liquid chromatography (Agilent, Santa Clara, CA) using a Bio-Rad guard column (P/N 125-0118, Bio-Rad Laboratories, Hercules, CA), Shodex Sugar SP0810 column (P/N F6378105, Showda Denko America, Inc., New York, NY) and refractive index detector.

Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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