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The cells were seeded into 96-well plates (5 × 103 cells/well) and treated for 72 hours (PANC-1) or 48 hours (MIA PaCa-2). The cell proliferation was measured using a CellTiter-Blue Cell Viability Assay Kit (Promega, Madison, WI) according to the manufacturer’s instructions. The stained cells were analyzed with a PerkinElmer EnSpire plate reader (PerkinElmer Inc., Waltham, MA).
Copyright and License information: ©2018 Saito et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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