Miniature Inhibitory Postsynaptic Currents (mIPSCs)

The miniature IPSCs (mIPSCs) were recorded in the voltage-clamp mode at a holding potential of −65 mV using a high chloride intracellular solution, containing 105 mM KCl instead of K-gluconate. To block AP triggered release, 0.5 μm tetrodotoxin was added to the aCSF. DNQX, AP-5, and {"type":"entrez-protein","attrs":{"text":"CGP55845","term_id":"875097176","term_text":"CGP55845"}}CGP55845 were added to the aCSF to antagonize AMPA-, NMDA-, and GABA_B receptor-mediated currents, respectively, and to record pharmacologically isolated GABA_AR-mediated mIPSCs. Neurons were allowed to equilibrate for at least 5 min prior to recordings. Frequency, amplitudes and kinetics of mIPSCs, rise time_10–90% and decay time_90–10%, were automatically analyzed during baseline conditions and in presence of DynA using Clampfit10.