4.2. LCD Light Exposure

The 661W cells were exposed to LCDs with luminance of 0,100, 200, and 300 nits for morphological observation and to determine the suitable luminance and duration for subsequent experiments. 661W cells were seeded on a 24-well plate at a density of 5 × 10⁴/well, which was then placed on a special framework over the LCD (Figure 9). To ensure a stable growth environment for 661W cells, the device was placed inside the CO₂ incubator. Temperature was maintained between 36.5 and 37.5 °C.

Schematic diagram of 661W cells seeded on a 24-well plate with the exposure of liquid crystal displays (LCD).