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Isolation of iDC and CD103+ DCs for gene expression analysis
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iDCs and CD103+ DCs were sorted from naive lungs of WT mice using a FACS Aria with the gating strategy shown in Figure 5. RNA was harvested from these cells and cDNA was amplified utilizing the Cells-to-ct kit (ThermoFisher) according to manufacturers instructions. Ifnar1 and Ifnlr message was determined by RT-qPCR as described above.
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