oxPAPC measurement in PBMC

Among the different oxPAPC, 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine (POVPC) and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphorylcholine (PGPC) were taken into consideration in this study because they have been previously identified as the most bioactive components.25 Measurement of POVPC and PGPC was obtained on an Agilent 1100 (Agilent Technologies, Santa Clara, CA, USA) mass spectrometer equipped with an electrospray ion source, as previously described.25 Quantification of the peak areas was performed by single-ion monitoring in the elution time range of 10–20 minutes using appropriate software. Authentic 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine, POVPC, and PGPC were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).