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Broth microdilution susceptibility testing.
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Bacterial inocula were quantified by serial dilution plating. Broth microdilution MIC testing was performed according to CLSI M07 (18) and M100 (19) guidelines in CAMHB for both cefepime and the combination of cefepime-enmetazobactam. Use of enmetazobactam at a fixed concentration of 8 μg/ml was derived from the exposure-response relationship described in an in vivo infection model (26). MIC panels were manufactured by Thermo Fisher Scientific (Oakwood Village, OH) and shipped frozen to participating laboratories.
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