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Databases and sequence analyses

MJ Mario Juhas
JA James W. Ajioka
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The B. subtilis genome sequence was obtained from the SubtiWiki (http://subtiwiki.uni-goettingen.de/) and BioCyc (http://bsubcyc.org/) databases. The E. coli K-12 MG1655 genome sequence was obtained from the E. coli K-12 project website (http://www.xbase.ac.uk/genome/escherichia-coli-str-k-12-substr-mg1655). The sequences of the standard DNA parts were obtained from the Registry of Standard Biological Parts (http://parts.igem.org/Main_Page). Sequencing was performed by Source Bioscience (Cambridge, UK). Sequences were compared employing TBLASTX, BLASTN [32], and position-specific iterated BLAST (PSI-BLAST) [33] from the National Center for Biotechnology Information (NCBI) website (http://ncbi.nlm.nih.gov).

Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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