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Measurement of cytokines (TNF-α, IL-6 and PGE2)
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TNF-α, IL-6 and PGE2 were measured in culture supernatants using specific ELISA kits. These kits were procured from BD Pharmingen (BD Biosciences, San Jose, USA) for TNF-α and IL-6 assays and from Abcam (Cambridge, UK) for the PGE2 assay. The cytokines were measured as described in the vendor’s protocols [19].
Copyright and License information: ©2019 Alnahdi et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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