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Ligand receptor interactions were monitored using the Beta Arrestin PathHunter eXpress system (DiscoverX) and carried out essentially as in ref. 48, with CHO cells stably overexpressing recombinant human LGR6 or GPR148 receptors. Briefly, cells were plated in 96-well plates 48 hours prior to experiments. Test compounds were incubated with cells for 1 hour at 37°C, and receptor activation was determined by measuring chemiluminescence using the PathHunter detection kit (DiscoverX).
Copyright and License information: ©2019 American Society for Clinical Investigation
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