The rat gastric tissue in each group was collected and opened along the greater curvature. After squeezing out the gastric content, the ulcerated stomachs (3 × 3mm) were washed twice in ice-cold saline, fixed in 10% (V/V) neutral buffered formalin for 5 days, embedded in paraffin wax, sliced into 5 μm thicknesses, deparaffinized in xylene, rehydrated in a decreasing concentration gradient of ethanol, and finally stained with Hematoxylin-Eosin (HE) solution for the examination of histopathology. The observation was conducted with an Olympus microscope-BX60 (Japan Olympus Company, Tokyo, Japan).
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