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Tibia and femur bones were harvested from male C57BL/6J male mice. Bone marrow was flushed from the tibia and femur bones with DMEM complete medium. Cells were cultured without disturbance for 24 h, were washed to remove non-adherent cells, and were supplied with fresh DMEM complete medium, with medium renewal every 3 days (Leroux et al., 2010; Su et al., 2014).
Copyright and License information: ©2019 Liu, Zheng, Zhou, Chen, Chen and Xiao.
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