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Statistical analyses were performed with JMP software (version 13, SAS Institute) and Prism software (version 7, GraphPad Software). We defined n as the number of experiments or treated animals. The statistical analysis was performed using an unpaired t test (two-tailed) or a one-way ANOVA. Post hoc comparisons were made using Tukey–Kramer’s honestly significant difference (HSD) test or Dunnett’s test. Data distribution was assumed to be normal, but this was not formally tested before parametric tests. A P value of <0.05 was considered significant.
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