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Chemicals such as sodium chloride (NaCl), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), inositol hexaphosphate (IHP), and bezafibrate (BZF), the highest purity available, were purchased from Sigma-Aldrich. All samples were prepared in 100 mM HEPES, pH 7.4 with double distilled water. IHP was used in a final concentration of 2 mM, BZF in 10 mM, and the NaCl concentration was 100 mM. All the allosteric effectors were added in a concentration to ensure saturation of the binding sites (of 60 μM protein).
Copyright and License information: ©2018 Schay et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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