3.9. Antimicrobial Evaluation

A disc diffusion assay was used to determine the antimicrobial activity of the compounds [31] with replication (n = 3). Staphylococcus aureus and Candida albicans served as target models for bacteria and fungi. A total of 100 µg of each compound was loaded onto 6-mm sterile circular filter-paper discs. The paper discs were left to air-dry. The dried paper discs were placed onto nutrient agar plates that had already been inoculated with a lawn of target microorganisms. After 24 h of incubation, the antimicrobial activity of the compounds was calculated.