Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.2. Determination of Anti-Toxoplasma gondii Antibodies
Ask a
question
Favorite
Sera were obtained from venous blood and stored at −20 °C until tested. Sandwich ELISA test kits EIA Toxoplasma IgG (Test-Line Clinical Diagnostics Ltd., Brno, Czech Republic) were used to evaluate serum samples for the presence of specific IgG antibodies to T. gondii. The index of positivity (IP) was calculated for each sample according to the formula: “IP = sample OD (optical density)/average cut-off OD”. Samples with IP >1.1 were considered positive.
Copyright and License information: ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
