2.6. Release Profile of Lactobacillus acidophilus

To investigate the release behavior of Lactobacillus acidophilus from the xanthan–chitosan capsules in gastro-intestinal fluid, 9 mL of brown milk beverage containing 1 g of Lactobacillus acidophilus microcapsules or 1 mL free Lactobacillus acidophilus suspension was incorporated into 40 mL SGF (pH = 1.5). The sample was incubated at 37 °C for 0, 0.5, 1, 1.5, and 2 h while shaking at 110 rpm. Subsequently, it was continuously transferred into SIF (pH of 6.8) for another 2 h. wet microcapsules were collected every 30 min and washed with sterilized saline water. They were then used for the enumeration of the viable count. A spectrophotometer was employed for release study. A 2.0 mL aliquot was collected and the absorbance at 600 nm was determined in the stipulated time (every 30 min).