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Plasmids and transfection

LL Lei Liu
YY Yi Yang
SL Shihua Liu
TT Tianyu Tao
JC Junchao Cai
JW Jueheng Wu
HG Hongyu Guan
XZ Xun Zhu
ZH Zhenjian He
JL Jun Li
ES Erwei Song
MZ Musheng Zeng
ML Mengfeng Li
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The SHCBP1 expression plasmid was generated by PCR subcloning the human SHCBP1 coding sequence into the lentiviral transfer plasmid pSin-puro (Clontech, Palo Alto, CA) to generate plasmid pSin-SHCBP1. To deplete SHCBP1 expression, two human shRNA sequences (sh1: GCGATTCAGAGCCTATCAA; sh2: CCATAGTGATCCATTGTCT) were cloned into the pSuper-retro-puro plasmid to generate pSuper-retro-SHCBP1-sh1 and pSuper-retro-SHCBP1-sh2. Human EGF-coding sequence was subcloned into the lentiviral transfer plasmid to generate plasmid pSin-EGF. Retroviral and lentiviral production and infection were performed according to the manufacturer’s instructions.

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