Identification of serum protein complexes of interest

Each gel band in native-PAGE gel was transferred into a 0.6 mL eppendorf tube, followed by the incubation for 45 min at room temperature in the equilibrium buffer (93.8 mM Tris-HCl, pH 6.8, 10% v/v glycerol, 2% w/v sodium dodecyl sulfate) including 3% (w/v) dithiothreitol, and then the band was incubated for 35 min at room temperature in the above-mentioned equilibrium buffer with 10% (w/v) iodoracetamide. The gel band was further separated using sodium dodecyl sulfate-PAGE. Each gel was run at 60 V for 1 h, followed by 120 V for 2 h. Gel bands from the sodium dodecyl sulfate-PAGE gel were excised and digested followed by the identification of the proteins of interest as described previously ¹¹.