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All candidate risk variants suggested by the statistical analysis were subjected to Sanger sequencing verification in the respective CRC cases. False risk alleles caused by incorrect genotyping were subsequently removed. Available family members of confirmed risk variant carriers were also tested for the same variant by Sanger sequencing to investigate variant segregation in the family.
Copyright and License information: ©2018 Jiao et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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