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Liquid chromatography-mass spectrometry (QTOF).

JR Jacob P. Richards
WC Wenlong Cai
NZ Nicholas A. Zill
WZ Wenjun Zhang
AO Anil K. Ojha
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LC-MS analysis was performed using an Agilent Technologies 6520 Accurate-Mass quadrupole time of flight (Q-TOF) LC-MS instrument and an Agilent Eclipse Plus C18 column (4.6 by 100 mm). A linear gradient of 2 to 98% acetonitrile (vol/vol) over 30 min in H2O with 0.1% formic acid (vol/vol) at a flow rate of 0.5 ml/min was used. HRMS/MS analysis was conducted using targeted MS/MS with collision energy of 20 V.

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