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1 μg of each peptide mixture were separated on a 23 minute gradient using an EASY-nLC II (Proxeon, Odense, Denmark) equipped with a 200 mm*75μm ID pulled-emitter column, packed with 3μm Reprosil Pur C18 material (Dr. Maisch, Germany), operating at 250 nL/min. Samples were analyzed using a Q-Exactive HF mass spectrometer (Thermo Scientific, Bremen, Germany). Data was recorded in a Top 10 DDA manner with an MS1 resolution of 120 000 and an MS2 resolution of 15 000.
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