EthoVision Video-Tracking Settings and Export

We tracked thrips behavior with EthoVision® XT 11.5 video tracking and analysis software (Noldus Information Technology BV, Wageningen, The Netherlands, www.noldus.com/ethovision) at 3.33 frames/s. Each leaf disc was assigned to a specific zone (Z1 and Z2); in addition, there was a neutral zone that did not contain leaf material (Z3). Dynamic subtraction and center-point detection were used as detection methods, with a dark contrast of 8–255. Subject size detection was limited to the range of 40–385 pixels. Pixel smoothing was set to medium. Moving thresholds were not set because EthoVision was only used for tracking and not for event analysis. Raw data files with genotypes in a “Genotype” column separated by a “$” sign were exported per subject for analysis in EthoAnalysis as .txt files for each arena (ca 1 Mb per arena per hour).