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Actinomycin D treatment and RNA stability analysis
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Control or RPSAP52-depleted A673 clones were treated with either 0.5% DMSO or 5 µg ml−1 Actinomycin D (Sigma) for 9 h. Pellets of each condition and treatment were harvested at different times and RNA was extracted for the RT-qPCR experiments. All data were normalized with respect to GUSB as endogenous control and gene expression fold-changes induced by Actinomycin D were calculated relative to the control (DMSO) cells of each condition and time point. c-FOS and GAPDH were used as controls of the experiment due to their short and long half-life, respectively.
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