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Cells were pretreated with 3-MA (3-methyladenine, Sigma, m-9281, USA, 5 mM)/CQ (chloroquine diphosphate, Sigma, C6628, USA, 10 μM)/BafA1 (bafilomycin A1, Selleck, S1413, China, 100 nM) for 1 h, followed by fluoxetine (10 μM) and corticosterone (10 μM) incubation for 24 h. Supernatants were discarded and H2DCF-DA (20 μM) was added to stain for 30 min at 37 °C. Cells were washed twice with D-hank’s solution, then fixated with 4% paraformaldehyde for 15 min and observed under a fluorescence microscope (Nikon).
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