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ELISA measurements

DL Denis Lane
IM Isabelle Matte
CL Claude Laplante
PG Perrine Garde-Granger
AC Alex Carignan
PB Paul Bessette
CR Claudine Rancourt
AP Alain Piché
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Cytokine levels in peritoneal fluid samples were determined by ELISA using the commercially available human Quantikine kits from R&D Systems (Minneapolis, MN). The assays were performed in duplicate according to the manufacturer’s protocols. The detection threshold was 1.1 ng/ml for CCL18. The intra-assay variability was 3.2–3.7 % for CCL18. The inter-assay variability was 3.5 %. All samples were examined in duplicate and the median values were used for statistical analysis.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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