Rapamycin Treatment and Survival Curve

Rapamycin (>98% purity) was purchased from Dalian Meilun Biotechnology Co. Ltd. (Cas:53123-88-9; China). Rapamycin was dissolved in ethanol at 10 mg/ml and diluted in 5% Tween-80 and 5% polyethylene glycol 400 (Solarbio Science and Technology Co., Ltd., China). For rapamycin treatment, 6 h after irradiation, mice were treated with rapamycin (4 mg/kg) by subcutaneous injection and then the injection was repeated every other day until day 7 after irradiation. Mice were monitored daily until day 30 after irradiation. As a control, mice were irradiated or non-irradiated and received subcutaneous injection of the same volume of vehicle (200 μl).