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Yeast one-hybrid assay (Y1H)

RJ Rong Jin
QZ Qing-gang Zhu
XS Xin-yue Shen
MW Miao-miao Wang
WJ Wajeeha Jamil
DG Donald Grierson
XY Xue-ren Yin
KC Kun-song Chen
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According to library screening results, the protein-DNA interaction was verified with DkNAC7-AD and DkERF9 promoter, individually. Meanwhile, interaction between DkNAC7 and DkPDC2 promoter was also investigated by Y1H. The promoter of DkERF9 was constructed into pAbAi vector (primers are listed in Table 1). The DkPDC2-pAbAi was constructed by Min et al. [8]. The full-length sequence of transcription factor DkNAC7 was subcloned into pGADT7 AD vector (AD) (primers are listed in Table 1).

Auto-activation and the interaction analysis were conducted according to the manufacturer’s protocol.

Copyright and License information:  ©2018 Jin et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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