2.6. Excision Wound Model

Excision wound was created as described previously [53]; five groups of animals each containing eight rats were shaved on the dorsum portion using depilatory cream (Reckitt Benckiser, Inc., UK) and anesthetized using ketamine hydrochloride (50 mg/kg, i.p., body weight) in combination with xylazine hydrochloride (10 mg/kg) of body weight [52]. On shaved dorsal region an impression was made and area of the wound to be created was marked. Using toothed forceps and pointed scissors, a full thickness excision wound with a circular area of 314 mm² and 2 ml in depth was created along the marking, and rats were left undressed to the open environment.